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To isolate protoplast, one needs:
a. pectinase
b. cellulase
c. both pectinase and cellulase
d. chitinase.

Solution: Plant cell walls are made up of cellulose, soluble proteins, and pectin, and are organized in layers. As a result, both pectinase (a pectin-degrading enzyme) and cellulase are required to isolate protoplast (the enzyme which degrades cellulose).  Alone pectinase and cellulase cannot help the isolation of protoplast.

So, the correct option is (c).