During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first? (1) Annealing (2) Extension (3) Denaturation (4) Ligation

Correct option:(3)Denaturation
Solution:

• High temperature about 94°C is required for the process of denaturation which is the first step of PCR.
• Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase.
• Annealing is performed at 50°-60°C which is the second step that can get affected.
• Addition of nucleotides to the primer, synthesizing a new DNA strand using only the template sequences with the help of enzyme
• DNA polymerase is called primer extension/ polymerisation.