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Agrobacterium tumefaciens is most widely used for gene transfer because __________ A) it causes crown gall tumours B) of its ability to insert Ti plasmid into nuclear genome C) it can grow anywhere D) it has ability to kill pathogenic bacteria

Correct option:(B)of its ability to insert Ti plasmid into nuclear genome

Solution:

  • Agrobacterium tumefaciens naturally infects the wound sites in dicots causing the crown gall disease.
  • It is capable of transferring T-DNA of the Tumour inducing (Ti) plasmid into the nucleus of infected cells.
  • It will then integrate into the host genome and is transcribed, causing the crown gall disease.
  • Property of tumor formation is exploited in biotechnology.
  • So, Agrobacterium tumefaciens has become the most used method for plant transformation by gene transfer.