Correct option:(3)Denaturation
Solution:
- High temperature about 94°C is required for the process of denaturation which is the first step of PCR.
- Ligation of DNA fragments is performed with the help of an enzyme called DNA ligase.
- Annealing is performed at 50°-60°C which is the second step that can get affected.
- Addition of nucleotides to the primer, synthesizing a new DNA strand using only the template sequences with the help of enzyme
- DNA polymerase is called primer extension/ polymerisation.