To isolate protoplast, one needs:
a. pectinase

b. cellulase

c. both pectinase and cellulase

d. chitinase.
To isolate protoplast, one needs:
a. pectinase

b. cellulase

c. both pectinase and cellulase

d. chitinase.

Solution: Plant cell walls are made up of cellulose, soluble proteins, and pectin, and are organized in layers. As a result, both pectinase (a pectin-degrading enzyme) and cellulase are required to isolate protoplast (the enzyme which degrades cellulose).  Alone pectinase and cellulase cannot help the isolation of protoplast.

So, the correct option is (c).